Serveur d'exploration Phytophthora

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Seed defensins of barnyard grass Echinochloa crusgalli (L.) Beauv.

Identifieur interne : 001B83 ( Main/Exploration ); précédent : 001B82; suivant : 001B84

Seed defensins of barnyard grass Echinochloa crusgalli (L.) Beauv.

Auteurs : Tatyana I. Odintsova [Russie] ; Eugene A. Rogozhin ; Yurij Baranov ; Alexander Kh Musolyamov ; Nasser Yalpani ; Tsezi A. Egorov ; Eugene V. Grishin

Source :

RBID : pubmed:18625284

Descripteurs français

English descriptors

Abstract

From the annual weed barnyard grass Echinochloa crusgalli (L.) Beauv., two novel defensins Ec-AMP-D1 and Ec-AMP-D2 that differ by a single amino acid substitution were isolated by a combination of different chromatographic procedures. Both defensins were active against several phytopathogenic fungi and the oomycete Phytophthora infestans at micromolar concentrations. The Ec-AMP-D1 showed higher activity against the oomycete than Ec-AMP-D2. The comparison of the amino acid sequences of the antifungal E. crusgalli defensins with those of earlier characterized T. kiharae defensins [T.I. Odintsova, Ts.A. Egorov, A.Kh. Musolyamov, M.S. Odintsova, V.A. Pukhalsky, E.V. Grishin, Seed defensins from T. kiharae and related species: genome localization of defensin-encoding genes, Biochimie, 89 (2007) 605-612.] that were devoid of substantial antifungal activity point to the C-terminal region of the molecule as the main determinant of the antifungal activity of E. crusgalli defensins.

DOI: 10.1016/j.biochi.2008.06.007
PubMed: 18625284


Affiliations:


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Le document en format XML

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<term>Amino Acid Sequence (MeSH)</term>
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<term>Anti-Infective Agents (isolation & purification)</term>
<term>Anti-Infective Agents (pharmacology)</term>
<term>Antifungal Agents (isolation & purification)</term>
<term>Antifungal Agents (pharmacology)</term>
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<term>Defensins (isolation & purification)</term>
<term>Defensins (pharmacology)</term>
<term>Echinochloa (chemistry)</term>
<term>Fungi (drug effects)</term>
<term>Fungi (metabolism)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Phytophthora infestans (drug effects)</term>
<term>Phytophthora infestans (metabolism)</term>
<term>Seeds (chemistry)</term>
<term>Sequence Alignment (MeSH)</term>
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<term>Antifongiques (pharmacologie)</term>
<term>Champignons (effets des médicaments et des substances chimiques)</term>
<term>Champignons (métabolisme)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Défensines (génétique)</term>
<term>Défensines (isolement et purification)</term>
<term>Défensines (pharmacologie)</term>
<term>Echinochloa (composition chimique)</term>
<term>Graines (composition chimique)</term>
<term>Phytophthora infestans (effets des médicaments et des substances chimiques)</term>
<term>Phytophthora infestans (métabolisme)</term>
<term>Substitution d'acide aminé (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
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<term>Defensins</term>
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<term>Antifungal Agents</term>
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<term>Anti-Infective Agents</term>
<term>Antifungal Agents</term>
<term>Defensins</term>
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<term>Echinochloa</term>
<term>Seeds</term>
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<term>Echinochloa</term>
<term>Graines</term>
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<term>Fungi</term>
<term>Phytophthora infestans</term>
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<term>Champignons</term>
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<term>Amino Acid Substitution</term>
<term>Molecular Sequence Data</term>
<term>Sequence Alignment</term>
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<term>Alignement de séquences</term>
<term>Données de séquences moléculaires</term>
<term>Substitution d'acide aminé</term>
<term>Séquence d'acides aminés</term>
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<div type="abstract" xml:lang="en">From the annual weed barnyard grass Echinochloa crusgalli (L.) Beauv., two novel defensins Ec-AMP-D1 and Ec-AMP-D2 that differ by a single amino acid substitution were isolated by a combination of different chromatographic procedures. Both defensins were active against several phytopathogenic fungi and the oomycete Phytophthora infestans at micromolar concentrations. The Ec-AMP-D1 showed higher activity against the oomycete than Ec-AMP-D2. The comparison of the amino acid sequences of the antifungal E. crusgalli defensins with those of earlier characterized T. kiharae defensins [T.I. Odintsova, Ts.A. Egorov, A.Kh. Musolyamov, M.S. Odintsova, V.A. Pukhalsky, E.V. Grishin, Seed defensins from T. kiharae and related species: genome localization of defensin-encoding genes, Biochimie, 89 (2007) 605-612.] that were devoid of substantial antifungal activity point to the C-terminal region of the molecule as the main determinant of the antifungal activity of E. crusgalli defensins.</div>
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